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TL1A, PerCP-eFluor™ 710, clone: Tandys1a, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-7911-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15311400

  • £206.01 / 100µg


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Description

Description

Description: The Tandys1a monoclonal antibody reacts with human and mouse TL1A (TNFSF15). TL1A is a member of the TNF ligand family and is expressed by endothelial cells, tissue macrophages, monocyte-derived dendritic cells, plasma cells and lamina propria-derived lymphocytes. Among peripheral blood cells, TL1A has been shown to be expressed predominantly by CD4+CCR9+ lymphocytes. Its expression has been shown to be induced by TNF alpha, IL-1 alpha and IFN gamma. TL1A binds DR3, which has been shown to be specifically upregulated in Th17 cells among CD4+ T cells. TL1A-deficient dendritic cells are weak in their ability to support Th17 cells in vitro and show decreased severity of EAE. In vivo, TL1A has been shown to be upregulated in tissue lymphocytes of Crohn's Disease and ulcerative colitis patients.Applications Reported: This Tandys1a antibody has been reported for use in flow cytometric analysis, and intracellular staining followed by flow cytometric analysis.Applications Tested: This Tandys1a antibody has been tested by flow cytometric analysis of normal human peripheral blood cells or mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

TL1A
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q5UBV8, O95150
MGC129934, MGC129935, TL1, TL1A, VEGI, VEGI192A
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
Tandys1a
PerCP-eFluor 710
TNFSF15
Conjugated
TNFSF15
IgG1, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
9966
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