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TIM-4, PerCP-eFluor™ 710, clone: 54 (RMT4-54), eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-5866-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15569286

  • £83.31 / 25µg


Estimated Shipment date
from Supplier 03-07-2018
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Description

Description

Description: The monoclonal antibody 54 (RMT4-54) recognizes mouse Tim-4 (T cell, Ig, mucin). Unlike other Tim family members, Tim-4 is not expressed on T lymphocytes but rather found on antigen presenting cells such as lymphoid and myeloid DC's and macrophages. Tim-4 has been shown to interact with Tim-1 thereby suggesting a role of Tim-4 in the balance of Th1/Th2 regulation. Additionally Tim-4 has been shown to be a receptor for phosphatidylserine and may play a role in engulfment of apoptotic cells. Expression is found on a subset of CD11c+ splenic cells.Applications Reported: This 54 (RMT4-54) antibody has been reported for use in flow cytometric analysis.Applications Tested: This 54 (RMT4-54) antibody has been tested by flow cytometric analysis of mouse resident peritoneal exudate cells. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

TIM4
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q6U7R4
SMUCKLER, TIM4, T-cell immunoglobulin and mucin domain-containing protein 4, T-cell membrane protein 4, TIM-4, TIMD-4
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
54 (RMT4-54)
PerCP-eFluor 710
Timd4
Conjugated
Timd4
IgG2a, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
276891
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