TIM-4, PE, clone: 54 (RMT4-54), eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-5866-82

Product Code. 15248859

Description

Description: The monoclonal antibody 54 recognizes mouse Tim-4 (T cell, Ig, mucin). Unlike other Tim family members, Tim-4 is not expressed on T lymphocytes but rather found on antigen presenting cells such as lymphoid and myeloid DC's and macrophages. Tim-4 has been shown to interact with Tim-1 thereby suggesting a role of Tim-4 in the balance of Th1/Th2 regulation. Additionally Tim-4 has been shown to be a receptor for phosphatidylserine and may play a role in engulfment of apoptotic cells. Expression is found on a subset of CD11c+ splenic cells.Applications Reported: This 54 (RMT4-54) antibody has been reported for use in flow cytometric analysis.Applications Tested: This 54 (RMT4-54) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Please consider the use of a viability dye like 7-AAD (cat 00-6993) when staining for Tim4.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: TIM4
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q6U7R4
• Gene Alias: SMUCKLER, TIM4, T-cell immunoglobulin and mucin domain-containing protein 4, T-cell membrane protein 4, TIM-4, TIMD-4
• Host Species: Rat
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Murine

• Applications: Flow Cytometry
• Clone: 54 (RMT4-54)
• Conjugate: PE
• Gene: Timd4
• Format: Conjugated
• Gene Symbols: Timd4
• Isotype: IgG2a, kappa
• Quantity: 100μg
• Storage Requirements: Store at 2-8°C. Do not freeze. Light-sensitive material.
• Primary or Secondary: Primary
• Gene ID (Entrez): 276891

For Research Use Only.