Invitrogen™ Human TrxR1, Recombinant Protein

Recombinant Protein

Brand:  Invitrogen™ LFP0021

Product Code. 10470270

Description

Thioredoxin Reductase 1 Recombinant Protein, LF-P0021, from Invitrogen.

The mammalian thioredoxin reductases (TrxRs) are a family of selenocysteine-containing pyridine nucleotide-disulfide oxido-reductases. All the mammalian TrxRs are homologous to glutathione reductase with respect to primary structure including the conserved redox catalytic site (-Cys-Val-Asn-Val-Gly-Cys-) but distinctively with a C-terminal extension containing a catalytically active penultimate selenocysteine (SeCys) residue in the conserved sequence (-Gly-Cys-SeCys-Gly). TrxR is homodimeric protein in which each monomer includes an FAD prosthetic group, a NADPH binding site and a redox catalytic site. Electrons are transferred from NADPH via FAD and the active-site disulfide to C-terminal SeCys-containing redox center, which then reduces the substrate like thioredoxin. The members of TrxR family are 55-58 kilodalton in molecular size and composed of three isoforms including cytosolic TrxR1, mitochondrial TrxR2, and TrxR3, known as Trx and GSSG reductase (TGR). TrxR plays a key role in protection of cells against oxidative stress and redox-regulatory mechanism of transcription factors and various biological phenomena. This protein corresponds to human Thioredoxin Reductase 1 (1-498 aa with additional 3 amino acids-GSH at N-term), which has a molecular weight of 55 kDa. Purity: >95% by SDS-PAGE. After reconstitution, store at -80°C and avoid freeze and thaw cycles. Reconstitute using 500 uL deionized water.

Specifications

• Accession Number: Q16881
• Formulation: 0.02M HEPES with 1mM EDTA and no preservative; pH 7
• Name: Human TrxR1
• Quantity: 500μg
• Storage Requirements: -20°C
• Common Name: Human TrxR1
• Specific Reactivity: Human
• Cross Reactivity: Hu
• Recombinant: Recombinant
• Protein Subtype: Recombinant

• For Use With (Application): Control
• Gene ID (Entrez): 7296
• pH Range: 7
• Source: E. Coli
• Regulatory Status: RUO
• Gene Symbol: TXNRD1
• Conjugate: Unconjugated
• Species: E. coli
• Form: Lyophilized
• Purity or Quality Grade: purified