Thermo Scientific™ ThermoPrme™ TaqDNA Polymerase ReddyMx™ PCR Kts and Master Mxes

Thermo Scientific™ ReddyMix formats save time by allowing for direct gel loading post-PCR.

Brand:  Thermo Scientific™ AB0790B

Product Code. 11781588

Description

Save time with direct gel loading post-PCR using Thermo Scientific™ ThermoPrime™ Taq DNA Polymerase ReddyMix™ PCR Kits and Master Mixes.

ReddyMix buffers contain an inert red dye that eases PCR setup and aids in error-free pipetting, the precipitant allows the completed PCR reaction to be directly loaded onto agarose gels. PCR assays using standard Taq DNA polymerase and basic buffer formulations are subject to a loss in polymerase activity over the multiple high temperature steps of PCR cycling. ThermoPrime Taq DNA Polymerase has enhanced thermal stability to prevent this loss of activity. A proprietary buffer component protects ThermoPrime during high temperature steps, ensuring optimal performance throughout the reaction

• Enhanced enzyme stability for increased yield
• Advanced buffering system minimizes PCR optimization steps
• Convenient Master Mix formats save time and reduce the risk of sample contamination during assay setup
• Enhanced enzyme stability for increased yield over standard Taq DNA polymerase formulations

ThermoPrime is available in a range of ReddyMix formats and pack sizes to suit any workflow.

Format Options:

• ReddyMix Enzyme Kits are the most basic format. Kits include the enzyme, ReddyMix PCR buffer, and 25mM MgCl₂ solution, each in separate vials. dNTPs must be purchased separately

• ReddyMix Complete PCR Kits include all the components contained in the ReddyMix Enzyme Kit, plus they have HPLC-purified dNTP mix. Complete PCR kits are a convenient option that provides all the components necessary for PCR

• ReddyMix Master Mix formats consist of a single ReddyMix master buffer containing all the components needed for PCR. Master Mixes are the most convenient format and significantly reduce assay setup steps

• Premixed MgCl₂ Kits have MgCl₂ premixed into the PCR buffer and will be at 1.5mM concentration in the final reaction

• Red Taq/Red Hot Indicator Dye Kits include an inert red dye in the enzyme mix that helps verify enzyme addition during reaction setup

Compatible with:

Thermo-Prime Taq Kits and Master Mixes

Recommended for:

Amplifying fragments up to 5kb; PCR; RT-PCR; Sequencing; Genotyping; Library Construction; Cloning; Pathogen Detection; Single Cell PCR

Note:

The inert red dye migrates between bromophenol blue and xylene cyanol, at approximately 300 bp, depending on the agarose concentration.

Specifications

• Content And Storage: Store at -20°C for long-term storage. Stable at 4°C for up to four weeks. Avoid freeze-thaw cycles.
• Quantity: 5U/µL
• No. of Reactions: 4000 x 25µL Rxns

• Description: ThermoPrime DNA Polymerase Enzyme Kit w/ReddyMix Buffer
• For Use With (Application): Standard PCR, RT-PCR, DNA Sequencing, Genotyping, Pathogen Detection

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.