Thermo Scientific™ Thermo-Start™ Taq DNA Polymerase PCR Kts and Master Mxes

Hot-start modified for PCR applications requiring maximum specificity and sensitivity to detect rare or complex targets and allow for room-temperature reaction setup.

Brand: Thermo Scientific™

Manufacturer Part Number: AB-0908/B


UNSPSC: 41106300

Code: LE

Additional Details:
Additional Details: Weight: 0.15000kg

Disclaimers: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Product Code. 11865222

Quantity Price
1 £1094.00 / 2500 units
EU Stock 30
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Description and Specification


Compatibility Thermo-Start Taq Reddymix Kits and Master Mixes
Concentration 5U/µL
Description Thermo-Start DNA Polymerase Enzyme Kit
For Use With (Application) Amplifying fragments up to 5kb; Hot-Start PCR; RT-PCR; Sequencing; Multiplexing; Genotyping; Library Construction; Cloning; Pathogen Detection; Single Cell PCR
Quantity 4000 x 25µL Rxns
Storage Requirements Store at -20°C for long-term storage. Stable at 4°C for up to four weeks. Avoid freeze-thaw cycles.

Thermo-Start Taq DNA Polymerase is a hot-start enzyme combined with an optimized buffer system that gives superior specificity and sensitivity. The chemically modified Thermo-Start Taq remains completely inactive until it is switched on by a high temperature incubation step at the start of the PCR reaction. This prevents nonspecific amplification during reaction setup, eliminating smearing and the formation of nontarget bands.
  • Hot-Start modification ensures stringent specificity
  • Safe for room temperature reaction setup
  • Enhanced enzyme stability for increased yield and sensitivity
  • Advanced buffering system minimizes PCR optimization steps
  • High Performance Buffer formulation for GC-rich and other complex templates
Thermo-Start is available in a range of formats and pack sizes to suit any workflow.

Format Options:

  • Enzyme Kits are the most basic format. Kits include the enzyme, PCR buffer, and 25mM MgCl2 solution, each in separate vials. dNTPs must be purchased separately

  • Complete PCR Kits include all the components needed to perform PCR, including an HPLC-purified dNTP mix. Complete PCR kits are a convenient option that still allows for MgCl2 optimization

  • Master Mix formats consist of a single master buffer containing all the components needed for PCR. Master Mixes are the most convenient format as they significantly reduce assay set-up steps and minimize the risk of sample contamination

  • Premixed MgCl2 Kits have MgCl2 premixed into the PCR buffer and will be at 1.5mM concentration in the final reaction

  • High Performance Buffer Kits are specially formulated for GC-rich or other complex targets with a high degree of secondary structure

Compatible with:

Thermo-Start Taq Reddymix Kits and Master Mixes

Recommended for:

Amplifying fragments up to 5kb; Hot-Start PCR; RT-PCR; Sequencing; Multiplexing; Genotyping; Library Construction; Cloning; Pathogen Detection; Single Cell PCR


Technical documents