RuvA Recombinant Protein, Invitrogen™

RuvA Recombinant Protein, RP-4906, from Invitrogen™

Overview
Brand: Invitrogen

Manufacturer Part Number: RP-4906

RUVA

UNSPSC: 12352203

Code: LD

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 11514191

Quantity Price
1 £ 638.0 / 20µg
Estimated Shipment date
from Supplier 22-03-2017
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Description and Specification

Specification

Conjugate Unlabeled
For Use With (Application) Control, Western Blot
Formulation 20μg purified protein (2.7mg/mL) in TBS with 50% glycerol, 5mM beta mercaptoethanol, 2mM EDTA and no preservative; pH 7.5
Host Species E. Coli
Storage Requirements store at -20°C, Avoid Freeze/Thaw Cycles
Molecular Weight ∽22
Recombinant Yes
Concentration 2.7mg/mL
Source E. coli
Format Purified
Name RuvA Recombinant Protein
Quantity 20μg
pH Range 7.5
Purification Method Chromatography

E. coli RuvA protein binds specifically to the Holliday structure which is the intermediate of recombination at the late stage of homologous recombination and recombination repair and forms a complex with RuvB motor protein allowing the migration of Holliday junction using ATP hydrolysis energy and expands the heteroduplex region. In solution, it forms a tetramer and binds to the cross-like DNA of the Holliday junction from below and above holding it in between.
Host Species: E. Coli
Formulation: 20μg purified protein (2.7mg/mL) in TBS with 50% glycerol, 5mM beta mercaptoethanol, 2mM EDTA and no preservative; pH 7.5
RP-4906 can be used as a positive control in Western blotting, as well as in studies on homologous recombination mechanism. RP-4906 calculated molecular weight is ∽22 kDa. Purity is 90%.