LexA Repressor Recombinant Protein, Invitrogen™

LexA Repressor Recombinant Protein, RP-4905, from Invitrogen™

Overview
Brand: Invitrogen

Manufacturer Part Number: RP-4905

LEXA REPRESSOR

UNSPSC: 12352203

Code: LD

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 11504191

Quantity Price
1 £ 1856.0 / 100µg
Estimated Shipment date
from Supplier 05-01-2017
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Description and Specification

Specification

Conjugate Unlabeled
For Use With (Application) Control, Western Blot
Format Purified
Formulation 100μg purified protein (0.8mg/mL) in TBS with 50% glycerol, 5mM beta mercaptoethanol, 2mM EDTA and no preservative; pH 7.5
Molecular Weight 23
Name LexA Repressor
pH Range 7.5
Quantity 100μg
Recombinant Yes
Source E. coli
Storage Requirements store at -20°C, Avoid Freeze/Thaw Cycles

E. coli LexA protein inhibits the transcription of the genes belonging to the SOS regulon that are related to DNA repair and cell division by recognizing and binding to the SOS-box sequence. LexA‘s self-protease activity is promoted by RecA protein which, responding to DNA damage, is activated by its binding to single-strand DNA accumulated in the cells. It is cleaved into two fragments and loses its function as a repressor. As the result, the expression of genes belonging to the SOS regulon is induced, and mutagenic activity in the cells are enhanced.
Host Species: E. Coli
Formulation: 100μg purified protein (0.8mg/mL) in TBS with 50% glycerol, 5mM beta mercaptoethanol, 2mM EDTA and no preservative; pH 7.5
RP-4905 can be used as a positive control in Western blotting to confirm that the Bait construct is expressed stably in the nucleus, as well as in studies on the mechanism of E. coli SOS response. RP-4905 calculated molecular weight is ∽23 kDa. Purity is 90%.