Invitrogen™ Human GSR, Recombinant Protein

Recombinant Protein

Brand:  Invitrogen™ LFP0024

Product Code. 10500861

Description

Glutathione Reductase (Hu) Recombinant Protein, LF-P0024, from Invitrogen.

Glutathione reductase (GR) is a member of pyridine nucleotide-disulfide oxidoreductases, which includes the closely related enzymes thioredoxin reductase, lipoamide dehydrogenase, trypanothione reductase and mercuric ion reductase. GR is a cytoplasmic flavoenzyme widely distributed in aerobic organisms. The dimeric protein is composed of two identical subunits, each containing 1 FAD and 1 redox-active disulfide/dithiol as components of the catalytic apparatus. It plays a role in maintaining glutathione (GSH) in its reduced form by catalyzing the reduction of glutathione disulfide (GSSG) : GSSG + NADPH + H+ to 2GSH + NADP+ In most eukaryotic cells, GR maintains the ratio of [GSH]/[GSSG] elevated, and participates in several vital functions such as the detoxification of reactive oxygen species as well as protein and DNA biosynthesis. This protein corresponds to human Glutathione Reductase (44-522 aa with additional 3 amino acids-GSH at N-term), which has a molecular weight of 51.7 kDa. Specific activity: 19.4 U/mg, where one unit will reduce 1 umol of oxidized glutathione per min at pH 7.5 at 25°C. Purity: >95% by SDS-PAGE. After reconstitution, store at -80°C and avoid freeze and thaw cycles.

Specifications

• Accession Number: P00390
• Formulation: 0.02M HEPES with no preservative; pH 7.5
• Name: Human GSR
• Quantity: 0.5mg
• Storage Requirements: -20°C
• Common Name: Human GSR
• Specific Reactivity: Human
• Cross Reactivity: Hu
• Recombinant: Recombinant
• Protein Subtype: Recombinant

• For Use With (Application): Control
• Gene ID (Entrez): 2936
• pH Range: 7.5
• Source: E. coli
• Regulatory Status: RUO
• Gene Symbol: GSR
• Conjugate: Unconjugated
• Species: E. coli
• Form: Lyophilized
• Purity or Quality Grade: purified

in vitro research only.