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Thermo Scientific™ Pierce™ Anti-c-Myc Magnetic Beads

Magnetic beads for recombinant c-Myc-tagged protein purification.

Brand:  Thermo Scientific™ 88842

Code : NEW

Additional Details : Weight : 3.62870kg

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Product Code. 15255733

  • £308.85 / 1mL


EU Stock 15
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Description

Description

Thermo Scientific Pierce Anti-c-Myc Magnetic Beads are affinity particles for immunoprecipitation of recombinant c-Myc-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators.

The blocked magnetic bead surface is coated with anti-c-Myc antibody, a highly specific mouse IgG1 monoclonal antibody (clone 9E10) that recognizes the c-Myc-epitope tag (EQKLISEEDL) derived from the human c-myc oncogene (p62 c-myc). The Pierce Anti-c-Myc Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms, such as the Thermo Scientific™ KingFisher™ Instruments, for high-throughput workflows.

Highlights:

  • Specific - highly specific anti-c-Myc monoclonal antibody (clone 9E10) enables high yield and high purity immunoprecipitation
  • Convenient and fast - product instructions provide an easy-to-follow, optimized protocol for immunoprecipitation in approximately one hour
  • Low non-specific binding - stable, pre-blocked beads and specific antibody minimize off-target binding for c-Myc-tag IP or co-IP experiments
  • Versatile - beads are compatible with manual and automated workflows (e.g., Thermo Scientific KingFisher Instruments)
Product Details:
Pierce Anti-c-Myc Magnetic Beads are convenient for the immunoprecipitation (IP) of recombinant c-Myc tagged proteins and the co-immunoprecipitation (Co-IP) of their interacting proteins. The beads are incubated with a cell lysate containing c-Myc tagged protein and the fusion protein is captured. The beads are subsequently washed and then the target proteins are eluted using 0.1M glycine (pH 2.0) 50mM NaOH, or SDS-PAGE sample buffer, the protocol has been optimized for each of these conditions. Anti-c-Myc antibody can be used to detect c-Myc tagged protein by Western blot analysis.

Specifications

Specifications

10mg/mL
Anti-c-Myc Magnetic Beads
Binding at least 100μg GST-c-Myc (26kDa fusion protein)/mL of bead suspension
High-affinity mouse IgG1 monoclonal antibody covalently coupled to blocked superparamagnetic beads; supplied at 10mg/mL in PBS containing 0.05% Tween-20 and 0.05% NaN3
1mL
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Special Offers

Special Offers

  • Big discounts are available on a range of popular products including chemicals, lab essentials, equipment, consumables, life science and safety products.
    : 13107

    2018-05-31

    *Discount will apply to qualifying orders received by Fisher Scientific between 03/04/2018 and 31/05/2018. Promotion code must be presented at the time of order. Discount applies to the list price in effect at the time the order is received. Promotion cannot be combined with other discounts or promotions. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.

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