Thermo Scientific™ Phusion™ U Green Hot Start DNA Polymerase

Thermo Scientific Phusion U DNA Polymerase is a proofreading enzyme (25x Taq) with a proprietary mutation to read through uracils and incorporate dUTP

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: F-556L

Phusion U Green HS Polymerase

UNSPSC: 41106300

Code: NEW

Additional Details:
Additional Details: Weight: 0.06000kg



Product Code. 15250143

Quantity Price
1 £ 520.0 / 500 units
Estimated Shipment date
from Supplier 13-12-2016
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Description and Specification

Specification

Concentration 2U/μL
For Use With (Application) Amplification of bisulphite-converted DNA, Amplification of damaged or aged DNA, Carry-over contamination control, Uracil-excision based (USER) cloning methods
Includes Phusion U Hot Start DNA Polymerase (2 U/μL), 5X Phusion Green HF Buffer, 5X Phusion Green GC Buffer, DMSO. Both Phusion Green HF and Phusion Green GC Buffer provide 1.5 mM MgCl2 in the final 1X concentration.
Quantity 500 Units
Storage Requirements Store at -20deg.C

Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology. Due to a proprietary mutation in the so called dUTP binding pocket of Phusion, Phusion U overcomes an important limitation of proofreading enzymes - it is able to incorporate dUTP and read through uracil present in DNA templates.
In addition to uracil usage possibility, Phusion U features all the superior properties of other Phusion DNA Polymerases - great accuracy, speed, ability to amplify long amplicons up to 20 kb and a high specificity with Affibody based hot start . These features make Phusion U Hot Start DNA Polymerase an excellent choice for such important applications as amplification of bisulphite-converted or damaged DNA as well as use of carryover contamination control.
Phusion U Hot Start DNA Polymerase is also available in the Master Mix and Green formats. Phusion U Hot Start PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. Only template and primers need to be added by the user. Phusion U Green format is a combination of Phusion U Hot Start DNA Polymerase and 5X Phusion Green Buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with Phusion U performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.
Highlights
Accuracy - extremely high fidelity DNA polymerase (25x Taq)
Uracil-tolerance - engineered to incorporate dUTP and amplify uracil containing templates
Specificity - hot start for reduced nonspecific amplification and primer degradation.
Speed - short extension times (15-30 s/kb)
• Direct loading of PCR products on gels
Applications
• Amplification of bisulphite-converted DNA
• Amplification of damaged or aged DNA
• Carry-over contamination control
• Uracil-excision based (USER) cloning methods
Includes
Phusion U Hot Start DNA Polymerase (F-555)
• Phusion U Hot Start DNA Polymerase, 2 U/μL
• 5X Phusion HF Buffer
• 5X Phusion GC Buffer
• DMSO
Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1X concentration.
Phusion U Green Hot Start DNA Polymerase (F-556)
• Phusion U Hot Start DNA Polymerase, 2 U/μL
• 5X Phusion Green HF Buffer
• 5X Phusion Green GC Buffer
• DMSO
Both Phusion Green HF and Phusion Green GC Buffer provide 1.5 mM MgCl2 in the final 1X concentration.
Phusion U Hot Start PCR Master Mix
• 2X Phusion U PCR Master Mix (Phusion U Hot Start DNA Polymerase, 2X reaction buffer, 3 mM MgCl2, 400 μM of each dATP, dTTP, dCTP, and dGTP)
• Water, nuclease-free

Hazardous:No
> Storage buffer:20 mM Tris-HCl (pH 7.4 at 25°C), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, stabilizers, and 50 % glycerol.
> Storage Condition:-20°C

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