Thermo Scientific™ MagJET™ Viral DNA and RNA Kit

Purifies viral nucleic acids from human and animal samples, including plasma, serum, saliva, and urine, using magnetic bead technology amenable to high-throughput operations.

Marque:  Thermo Scientific™ K2781

Code produit. 13575180

Description

The Thermo Scientific MagJET Viral DNA and RNA Kit is designed for fast and efficient purification of viral nucleic acids from various human and animal liquid samples such as plasma, serum, saliva and urine, as well as from nasal, buccal and urogenital swabs and blood.
The kit utilizes paramagnetic bead technology enabling high yields and robust performance. High binding capacity, uniform particle size, and rapid magnetic response of MagJET magnetic beads makes the technology ideal for high throughput automatic nucleic acid purification, as well as for manual nucleic acid purification by low sample throughput users.
The resulting high quality purified nucleis scids is free of proteins, nucleases and other contaminants or inhibitors, can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR and other enzymatic reactions.
Highlights
•Versatile - compatible with human serum, plasma, swabs, urine, saliva and other bodily liquids.
•High yields in wide linear range
•High purity - nuclease-, protein-, qPCR and RT-qPCR inhibitors-free
•Flexible - easily adjustable for high throughput automation platforms and manual processing
Applications
Downstream use in qualitative and quantitative nucleic acid based assays, e.g. (RT)-PCR, (RT)-qPCR
Includes
• Lysis Buffer
• MagJET Magnetic Beads
• Proteinase K
• Carrier RNA
• Wash Buffer 1 (conc.)
• Wash Buffer 2 (conc.)
• Water, nuclease free

Hazardous:Yes
> Hazardous::No
> Quality Control:The MagJET Viral DNA and RNA Kit has been tested by isolating DNA and RNA from 200 μL of human plasma spiked with plasmid DNA and RNA transcript. Yield of nucleic acids was evaluated by RT-qPCR and qPCR, and compared with spiked DNA (linearized plasmid DNA, 10⁵ copies/sample) and RNA (RNA transcript, 10⁶ copies/sample) copy number.
> Shelf Life:36 Months
> Shipping Condition:Ambient
> Storage Condition:Ambient

Spécification

• Content And Storage: Store Carrier RNA in the original aluminum bag at -20deg.C. Once open, store Proteinase K solution at -20deg.C. MagJET Magnetic Beads should be stored at 4deg.C. Other components of the kit should be stored at room temperature (15deg. to 25deg.C).
• For Use With (Application): Downstream use in qualitative and quantitative nucleic acid based assays like RT-PCR, RT-qPCR
• Includes: Lysis Buffer, MagJET Magnetic Beads, Proteinase K, Carrier RNA, Wash Buffer 1 (conc.), Wash Buffer 2 (conc.), nuclease free water
• No. of Reactions: 96 Preps