Thermo Scientific™ Luminaris HiGreen qPCR Master Mix

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Brand:  Thermo Scientific™ K0991

Product Code. 13575260

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.

Includes: Luminaris HiGreen qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer; Nuclease-Free Water

Description

• UDG in the master mix to prevent carry-over contamination
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols
• Excellent qPCR performance
• Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
• Sensitivity: single copy detection
• Wide linear range: accurate quantification across 9 orders of magnitude
• Reproducibility and convenience: ready-to-use 2X master mix

See Product Certificates

Specifications

• Detection Method: Detection Method: SYBR, PCR Method: qPCR
• For Use With (Equipment): Bio-Rad CFX384 Touch™, BioRad CFX96™, Cepheid SmartCycler, Corbett Rotor-Gene, Eppendorf MasterCycler RealPlex, MJ Chromo4, MJ Opticon, Roche LightCycler 480
• No. of Tests: 250 Reactions
• Sample Type: DNA, dsDNA
• Concentration: 2X

• For Use With (Application): Real time PCR (qPCR), gene expression analysis, siRNA validation, genotyping and pathogen detection
• Includes: Luminaris HiGreen qPCR Master Mix (2X) includes Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer; supplied with nuclease-free water.
• Quantity: 250 x 20μL rxns
• Storage Requirements: Store at -20°C.