Thermo Scientific™ Luminaris HiGreen qPCR Master Mix, Fluorescein

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Brand:  Thermo Scientific™ K0981

Product Code. 13545260

Includes:

• Luminaris HiGreen Fluorescein qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer. The master mix contains fluorescein passive reference dye for specific real-time platfo

Description

• UDG in the master mix to prevent carry-over contamination
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols
• Excellent qPCR performance
• Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
• Sensitivity: single copy detection
• Wide linear range: accurate quantification across 9 orders of magnitude
• Reproducibility and convenience: ready-to-use 2X master mix

Specifications

• Detection Method: Detection Method: SYBR, PCR Method: qPCR
• For Use With (Equipment): BioRad MyiQ, BioRad iCycler iQ, BioRad iQ5
• No. of Tests: 250 Reactions
• Sample Type: DNA, dsDNA
• Concentration: 2X

• For Use With (Application): Real time PCR (qPCR), gene expression analysis, siRNA validation, genotyping and pathogen detection
• Includes: Luminaris HiGreen Fluorescein qPCR Master Mix (2X) includes Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer. The master mix contains fluorescein passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied nuclease-free water.
• Quantity: 250 x 20μL rxns
• Storage Requirements: Store at -20°C.