Thermo Scientific™ Buffers for GeneJET™ Blood RNA Kit

Purify high quality total RNA from fresh or frozen whole blood, as well as blood collected in PAXgene and Tempus Tubes

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: R2861

Wash Buf2/GeneJET Blood RNA

UNSPSC: 41105500

Code: NEW

Additional Details:
Additional Details: Weight: 0.06000kg



Product Code. 15285106

Quantity Price
1 £ 34.95 / 25mL
Estimated Shipment date
from Supplier 09-12-2016
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Description and Specification

Specification

For Use With (Equipment) GeneJET Blood RNA Kit
Quantity 25mL
Storage Requirements Store at room temperature

The GeneJET™ Stabilized and Fresh Whole Blood RNA Kit is designed for rapid and efficient purification of high quality total RNA from various whole blood samples: fresh, frozen, or human blood samples stabilized with commercial stabilizers such as Tempus™ and PAXgene™.
The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The kit includes a DNase l for the enzymatic removal of genomic DNA. RNA can be isolated from whole blood in less than 30 minutes (including cell lysis and DNase l treatment).
Highlights
Versatile - compatible with fresh and frozen blood, buffy coat, as well as blood collected in PAXgene™and Tempus ™ Tubes
Convenient - specially optimized Lysis Buffer is suitable for RNA stabilization up to 48 h
Efficient - up to 4 μg of total RNA from 500 μL of healthy human blood
Pure - isolates intact total RNA free of any RT-qPCR inhibitors
Applications
Isolated total RNA can be used in:
• RT-PCR
• RT-qPCR
• Northern Blotting
Includes
• Lysis Buffer
• Wash Buffer 1 (conc.)
• Wash Buffer 2 (conc.)
• DNase l (lyophilized)
• DNase I Reconstitution Buffer
• 2x DNase I Buffer
• Manganese Chloride Solution
• Water, nuclease-free
• GeneJET RNA Purification Columns pre-assembled with Collection Tubes
• Collection Tubes, 2 mL
• Collection Tubes, 1.5 mL

Hazardous:No
> Quality Control:Thermo Scientific GeneJET Stabilized and Fresh Whole Blood RNA Purification Mini Kit is qualified by isolating total RNA from 250 μL of human whole blood following the protocol outlined in the manual. The quality of isolated RNA is evaluated spectrophotometrically and by agarose gel electrophoresis. The purified RNA has an A260/A280 ratio between 1.9 and 2.1 and the RNA integrity number (RIN) of ≥ 8.
> Shelf Life:24 Months
> Shipping Condition:Ambient
> Storage Condition:Ambient