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Themis, PE, clone: 1TMYS, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-5918-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15507056

  • £80.80 / 25µg


EU Stock 4
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Description

Description

Description: This 1TMYS monoclonal antibody reacts with mouse Thymocyte-expressed molecule involved in selection (Themis), also known as Grb2-associating protein (Gasp). Themis is a cytoplasmic protein that is essential for positive selection and the development of single-positive thymocytes. Highest levels of expression are found in CD4+CD8+ double-positive thymocytes, with lower expression found in mature T cells. In addition to Grb1, Themis has been found to associate with PLC gamma 1 and LAT and is quickly phosphorylated by SLP-76 following TCR signaling.There are 5 isoforms of mouse Themis that are produced as a result of alternative splicing. The 1TMYS antibody recognizes isoforms 1, 2, 3, and 4, but does not recognize isoform 5.Applications Reported: This 1TMYS antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 1TMYS antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes or splenocytes using the intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Themis
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q8BGW0
THEMIS, thymocyte selection associated, C6orf190, C6orf207, FLJ40584, MGC163388, TSEPA, bA325O24.3, bA325O24.4, thymocyte selection pathway associated, thymocyte-expressed molecule involved in selection
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
1TMYS
PE
Themis
Conjugated
Themis
IgG2a, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
210757
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