TGF beta Rat anti-Human, Clone: eBioTB2F, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 14-9943-85

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.01000kg



Disclaimers: For Research Use Only.

Product Code. 15298257

Quantity Price
1 £ 164.47 / 500µg
Estimated Shipment date
from Supplier 04-05-2017
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Description and Specification

Specification

Monoclonal or Polyclonal Monoclonal
Storage Requirements Store at 2-8°C.
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Host Species Rat
Quantity 500μg
Antigen TGF beta
Concentration 0.5mg/mL
Applications ELISA Capture
Species Reactivity Human
Conjugate Unlabeled
Regulatory Status RUO
Format Purified
Isotype IgG2a
Primary or Secondary Primary
Clone eBioTB2F
Gene Alias TGFb, transforming growth factor

The eBioTB2F antibody reacts with Transforming Growth Factor-beta (TGF-β), a pleiotropic cytokine, exists in five isoforms, known as TGF-β1-5. Homologies between isoforms range from 70-80% but no homology exists to TGF-α. TGF-β1 is ubiquitous and the most abundant form found in lymphoid organs, while other isoforms are expressed in a more restricted distribution. The biologically active state of all isoforms are disulfide-linked homodimers. The heat- and acid- stable monomeric subunits have a length of 112 amino acids. The isoforms of TGF-β arise by proteolytic cleavage of longer precursors; the isoforms are derived from the carboxyterminal ends of these precursors. Isoforms isolated from different species are evolutionarily closely conserved and have sequence identities on the order of 98%. Mature human, porcine, simian, chicken and bovine TGF-β1 are identical and differ from mouse TGF-β1 in a single amino acid. TGF-β1 is produced in very high levels by platelets. Other cellular sources of TGF-β1 include macrophages, lymphocytes, endothelial cells, chondrocytes, and leukemic cells. TGF-β1 secretion can be induced by steroids, retinoids, EGF, NGF, vitamin D3, and IL-1. Activities of TGF-β1 include inhibition of cell growth for inhibitor for normal and transformed epithelial cells, endothelial cells, fibroblasts, neurons, and lymphoid cells and other hematopoietic cell types. TGF-β1 inhibits the proliferation of T cells and NK cells and downregulates the activities of activated macrophages. TGF-β1 blocks the anti-tumor activity of IL-2 – bearing lymphokine-activated killer (LAK) cells.

Recently, TGF-β1 has been found to have a critical role in the development of regulatory T cells. Dendritic cells exposed to tumors have been reported to secrete TGF-β1 and stimulate expansion of natural T reg cells. Moreover, TGF-β1 has been shown to act as a costimulatory factor for expression of Foxp3, leading to the differentiation of CD4+CD25+ Treg cells from peripheral CD4+CD25- progeny. TGF-β-induced regulatory T cells have been termed Ti-Treg.