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TdT, PE, clone: 19-3, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-5846-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15298849

  • £187.71 / 100µg


Estimated Shipment date
from Supplier 02-07-2018
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Description

Description

Description: This 19-3 monoclonal antibody recognizes human and mouse terminal-deoxynucleotidyl transferase (TdT), a 60kDa polymerase responsible for the template-independent addition of N-nucleotides at gene segment junctions of developing lymphocytes. Expression is found in the nucleus of immature lymphocytes but not in mature lymphocytes or non-lymphoid cells. Tdt plays a critical role during TCR and Ig gene rearrangement. Regulation of expression in the mouse thymus correlates with T cell selection; decreased or absent after positive selection. Additionally Tdt has been shown to be present in lymphoma or lymphoblastic leukemia cells.Applications Reported: This 19-3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 19-3 antibody has been tested by intracellular staining using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5521) followed by flow cytometric analysis of mouse thymocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

TdT
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P04053, P09838
TDT
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
19-3
PE
DNTT
Conjugated
DNTT
IgG2b, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
1791
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