missing translation for 'onlineSavingsMsg'
Learn More

TCR beta, PE-eFluor 610, clone: H57-597, eBioscience™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 61-5961-80

Code : NEW

Additional Details : Weight : 0.09500kg

 View more versions of this product

Product Code. 15530547

  • £80.68 / 25µg


Estimated Shipment date
from Supplier 28-06-2018
Add to basket

Description

Description

Description: The H57-597 monoclonal antibody reacts with the beta chain of mouse TCR. TCR beta is expressed by thymocytes in a developmentally regulated manner and a majority of peripheral T cells. Crosslinking of the TCR complex with H57-597 induces activation and proliferation of T cells or apoptosis based on assay conditions. H57-597 is used as a phenotypic marker for TCR beta expressing T cells.Applications Reported: This H57-597 antibody has been reported for use in flow cytometric analysis.Applications Tested: This H57-597 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

TCR beta
Monoclonal
0.2mg/mL
PBS with 0.09% sodium azide; pH 7.2
Conjugated
Tcrb
IgG
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
21577
Flow Cytometry
H57-597
PE-eFluor 610
Tcrb
TCRbeta, Tib
Armenian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Videos

For Research Use Only.