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TCR beta, PE-Cyanine5, clone: H57-597, eBioscience™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 15-5961-83

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15350540

  • £229.79 / 200µg


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Description

Description

Description: The H57-597 monoclonal antibody reacts with the beta chain of mouse TCR. TCR beta is expressed by thymocytes in a developmentally regulated manner and a majority of peripheral T cells. Crosslinking of the TCR complex with H57-597 induces activation and proliferation of T cells or apoptosis based on assay conditions. H57-597 is used as a phenotypic marker for TCR beta expressing T cells.Applications Reported: The H57-597 antibody has been reported for use in flow cytometric analysis.Applications Tested: The H57-597 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

TCR beta
H57-597
PE-Cyanine5
Conjugated
IgG
RUO
Primary
Murine
Flow Cytometry
0.2mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Armenian Hamster
200μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
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