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TCR beta, Functional Grade, clone: H57-597, eBioscience™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-5961-85

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15238717

  • £156.21 / 500µg


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Description

Description

Description: The H57-597 monoclonal antibody reacts with the beta chain of mouse TCR. TCR beta is expressed by thymocytes in a developmentally regulated manner and a majority of peripheral T cells. Crosslinking of the TCR complex with H57-597 induces activation and proliferation of T cells or apoptosis based on assay conditions. H57-597 is used as a phenotypic marker for TCR beta expressing T cells.Applications Reported: This H57-597 antibody has been reported for use in flow cytometric analysis. The H57-597 monoclonal antibody has also been reported for cross-linking the T cell receptor resulting in T cell activation, and for in vitro depletion.Applications Tested: The H57-597 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

TCR beta
Monoclonal
1mg/mL
PBS with no preservative; pH 7.2
Purified
Tcrb
IgG
500μg
Store at 2-8°C.
Primary
21577
Functional Assay
H57-597
Functional Grade
Tcrb
TCRbeta, Tib
Armenian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
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