TCR beta Monoclonal Antibody (H57-597), APC-eFluor™ 780, eBioscience™, Invitrogen™

Armenian Hamster Monoclonal Antibody

Product Code	Brand	Quantity
15321670	Affymetrix eBioscience 47-5961-80	25 μg
15331670	Affymetrix eBioscience 47-5961-82	100 μg

Description

Description: The H57-597 monoclonal antibody reacts with the beta chain of mouse TCR. TCR beta is expressed by thymocytes in a developmentally regulated manner and a majority of peripheral T cells. Crosslinking of the TCR complex with H57-597 induces activation and proliferation of T cells or apoptosis based on assay conditions. H57-597 is used as a phenotypic marker for TCR beta expressing T cells. Applications Reported: This H57-597 antibody has been reported for use in flow cytometric analysis. Applications Tested: This H57-597 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat.

TCR beta Monoclonal antibody specifically detects TCR beta in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: TCR beta
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: bTCR; FLJ22602; MGC117436; MGC22624; MGC23964; MGC71411; T cell receptor beta chain; t-cell antigen receptor; T-cell receptor; TCR beta; Tcra; Tcrb; TCRbeta; TCRD; Tib; TRA
• Gene Symbols: Tcrb
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Gene: Tcrb

• Clone: H57-597
• Applications: Flow Cytometry
• Conjugate: APC-eFluor 780
• Host Species: Armenian Hamster
• Target Species: Mouse
• Gene Accession No.: 0
• Gene ID (Entrez): 21577
• Isotype: IgG
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.