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Thermo Scientific™ PCR Master Mix (2X)

Optimize routin PCR with this highly thermostable DNA polymerase.

Brand:  Thermo Scientific™ K0171

71.33 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : 88

Additional Details : Weight : 1.51950kg

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Product Code. 11853913

  • £107.00 / 200 reactions
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Description

Description

A 950 bp single copy gene from human genomic DNA was amplified at a wide range of Mg2+ concentrations.
M - GeneRuler 100 bp Plus DNA Ladder

Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Recombinant Taq DNA Polymerase is the ideal tool for standard PCR of templates 5 kb or shorter.

PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.

Highlights

  • Thermostable -half life is more than 40 min at 95°C
  • Generates PCR products with 3'-dA overhangs
  • Supplied with two buffers -10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4. The latter allows for PCR at wide range of magnesium concentrations and decreases unspecific priming
  • Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
Specifications

Specifications

Contains:
• 4 x 1.25 mL PCR Master Mix (2X), which includes:
— Taq DNA polymerase (0.05 U/μL),
— reaction buffer,
— 4 mM MgCl2, and
— 0.4 mM of each dNTP
• 4 x 1.25 mL Nuclease-free water

Store at -20°C.
Taq DNA Polymerase
PCR Master Mix (2X)
1 X
3'-A
Low
Standard
200 Reactions
No
SuperMix or Master Mix
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For Research Use Only. Not for use in diagnostic procedures.