T-bet, clone: eBio39D (39D, 3-9D), eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-5824-80
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The eBio39D monoclonal antibody reacts with mouse, rat and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN-gamma, and T-Bet also regulates the expression of IFN-gamma, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN-gamma, T-Bet is also known to regulate the expression of IL-12Rbeta and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells.Applications Reported: This eBio39D (39D, 3-9D) antibody has been reported for use in immunoprecipitation, and immunoblotting (WB).Applications Tested: This eBio39D (39D, 3-9D) antibody has been tested by western blot analysis of Th1-polarized mouse CD4+ T cells and can be used at 2 μg/mL as a starting dilution for western blotting.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|T-PET, T-bet, TBET, TBLYM, T-box expressed in T cells, T-box protein 21, T-box transcription factor TBX21, T-cell-specific T-box transcription factor T-bet, transcription factor TBLYM|
|eBio39D (39D, 3-9D)|
|Store at 2-8°C.|
For Research Use Only.