T-bet, PE-Cyanine7, clone: eBio4B10 (4B10), eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 25-5825-82
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionCell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered. T-bet, also known as TBX21, is a T-cell specific transcription factor containing a single T-box DNA-binding domain. It controls the expression of the TH1 cytokine, interferon-gamma. Both Th1 and Th17 cells are crucial in immune regulation and autoimmune disease development. T-bet initiates Th1 lineage development from naive Th precursor cells and directs T cell differentiation to Th1 versus Th17. T-bet is cooperating with Stat4 (signal tranducer and activator of transcription 4) in programming chromatin architecture for Th1 gene expression. Genetic variations in T-bet are associated with susceptibility to aspirin-induced asthma and nasal polyps.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|T-PET, T-bet, TBET, TBLYM, T-box expressed in T cells; T-box protein 21; T-box transcription factor TBX21; T-cell-specific T-box transcription factor T-bet; transcription factor TBLYM|
|Human, Mouse, Rhesus Monkey|
|eBio4B10 (4B10), 4-B10)|
|4° C, store in dark, DO NOT FREEZE!|
|30009, 57765, 694044|
For Research Use Only.