T-bet, PE-Cyanine7, clone: eBio4B10 (4B10), eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 25-5825-82
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: The eBio4B10 monoclonal antibody reacts with mouse and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN gamma, and T-Bet also regulates the expression of IFN gamma, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN gamma, T-Bet is also known to regulate the expression of IL-12R beta and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells.Applications Reported: This eBio4B10 (4B10, 4-B10) antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This eBio4B10 (4B10, 4-B10) antibody has been tested by intracellular stainign and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Buffer Set (cat. 00-5523) and protocol. Please see Best Protocol section; (refer to Protocol B: One step protocol for intracellular (nuclear) proteins). This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|T-PET, T-bet, TBET, TBLYM, T-box expressed in T cells, T-box protein 21, T-box transcription factor TBX21, T-cell-specific T-box transcription factor T-bet, transcription factor TBLYM|
|Human, Murine, Rhesus Monkey|
|eBio4B10 (4B10), 4-B10)|
|IgG1, kappa, kappa|
|4° C, store in dark, DO NOT FREEZE!, handling and experimental procedures.|
|30009, 57765, 694044|
For Research Use Only.