Syk, clone: 4D10.1, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-6696-82
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: This 4D10.1 monoclonal antibody recognizes human Syk, a 72-kDa member of the Syk/ZAP-70 family of non-receptor protein tyrosine kinases. Syk is expressed most highly in B cells, with lower expression in immature thymocytes, mast cells, and platelets. Syk can also be detected in fibroblasts, epithelial cells, hepatocytes, endothelial cells, and neuronal cells. This protein is a major component of signaling cascades downstream of the B and T cell antigen receptors and plays an essential role in lymphocyte development. Upon recruitment to immunoreceptor tyrosine-based activation motifs (ITAMs) on the antigen receptors, Syk is activated by phosphorylation on multiple tyrosines. Once activated, Syk phosphorylates proteins such as phospholipase C gamma and BLNK/SLP-65. Finally, abnormal Syk expression has been linked to tumor cell migration and invasion in several cancers.Applications Reported: This 4D10.1 antibody has been reported for use in flow cytometric analysis and western blotting.Applications Tested: This 4D10.1 antibody has been tested by immunoblotting of reduced Ramos cell lysate. This can be used at less than or equal to 5 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|Protein tyrosine kinase p72Syk|
|Flow CytometryþWestern Blot|
|Store at 2-8°C.|
For Research Use Only.