Syk Monoclonal Antibody (4D10.1), APC, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 17-6696-42

Product Code. 15558176

Description

Description: This 4D10.1 monoclonal antibody recognizes human Syk, a 72-kDa member of the Syk/ZAP-70 family of non-receptor protein tyrosine kinases. Syk is expressed most highly in B cells, with lower expression in immature thymocytes, mast cells, and platelets. Syk can also be detected in fibroblasts, epithelial cells, hepatocytes, endothelial cells, and neuronal cells. This protein is a major component of signaling cascades downstream of the B and T cell antigen receptors and plays an essential role in lymphocyte development. Upon recruitment to immunoreceptor tyrosine-based activation motifs (ITAMs) on the antigen receptors, Syk is activated by phosphorylation on multiple tyrosines. Once activated, Syk phosphorylates proteins such as phospholipase C gamma and BLNK/SLP-65. Finally, abnormal Syk expression has been linked to tumor cell migration and invasion in several cancers. Applications Reported: This 4D10.1 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 4D10.1 antibody has been pre-titrated and tested by intracellular staining using the Foxp3 buffer system and flow cytometric analysis of normal peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL.

Syk Monoclonal antibody specifically detects Syk in Human samples. It is validated for Flow Cytometry.

Specifications

• Antigen: Syk
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P43405
• Gene Symbols: Syk
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 6850
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: 4D10.1
• Conjugate: APC
• Gene: Syk
• Gene Alias: DKFZp313N1010; EC 2.7.10.2; FLJ25043; FLJ37489; HGNC:11205; kinase Syk; KSYK; p72Syk; p72-Syk; ptk72; SP1; spleen associated tyrosine kinase; spleen tyrosine kinase; SYK; syk signaling; Sykb; tyrosine-kinase syk; tyrosine-protein kinase SYK
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2a κ

For Research Use Only.