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Syk, APC, clone: 4D10.1, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 17-6696-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15548176

  • £76.10 / 25 tests


EU Stock 8
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Description

Description

Description: This 4D10.1 monoclonal antibody recognizes human Syk, a 72-kDa member of the Syk/ZAP-70 family of non-receptor protein tyrosine kinases. Syk is expressed most highly in B cells, with lower expression in immature thymocytes, mast cells, and platelets. Syk can also be detected in fibroblasts, epithelial cells, hepatocytes, endothelial cells, and neuronal cells. This protein is a major component of signaling cascades downstream of the B and T cell antigen receptors and plays an essential role in lymphocyte development. Upon recruitment to immunoreceptor tyrosine-based activation motifs (ITAMs) on the antigen receptors, Syk is activated by phosphorylation on multiple tyrosines. Once activated, Syk phosphorylates proteins such as phospholipase C gamma and BLNK/SLP-65. Finally, abnormal Syk expression has been linked to tumor cell migration and invasion in several cancers.Applications Reported: This 4D10.1 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 4D10.1 antibody has been pre-titrated and tested by intracellular staining using the Foxp3 buffer system and flow cytometric analysis of normal peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Syk
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P43405
Protein tyrosine kinase p72Syk
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
4D10.1
APC
SYK
Conjugated
SYK
IgG2a, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
6850
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