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Survivin, eFluor 660, clone: STLALYV, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-9176-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15540087

  • £252.41 / 100 tests


EU Stock 2
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Description

Description

Description: This STLALYV monoclonal antibody recognizes human survivin (also known as Birc5). Survivin is a member of the inhibitor of apoptosis (IAP) family that also includes XIAP, c-IAP1, c-IAP2, and NAIP. Survivin functions to inhibit caspase activation, thereby leading to negative regulation of apoptosis. Survivin also plays a critical role in mitosis and cytokinesis where it is expressed only during G2/M and localizes to the mitotic spindle and is found at the cleavage furrow/midplate. Thus, survivin is highly expressed in tumors and in fetal tissue, but is absent in terminally-differentiated cells and non-proliferating cells. Deletion of survivin in developing thymocytes as well as in mature T cells showed that survivin is critical for cell division in lymphoid cells but not for inhibition of survival; the same is true for B lymphocytes.Applications Reported: This STLALYV antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This STLALYV antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Survivin (BIRC5)
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
O15392
API4, Apoptosis Inhibitor 4, Apoptosis inhibitor survivin, Baculoviral IAP repeat containing 5, BIRC 5, EPR 1, IAP4, SVV, TIAP
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
STLALYV
eFluor 660
BIRC5
Conjugated
BIRC5
IgG2b, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
332
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