Thermo Scientific™ SulfoLink™ Immobilization Kit for Peptides, 2 mL

Covalently immobilize cysteine-peptides or proteins (sulfhydryl-ligands) for affinity purification using iodoacetyl-activated agarose beads and columns.

Brand:  Thermo Scientific™ 44999

Product Code. 10710825

Description

Thermo Scientific™ SulfoLink Coupling Resin is iodoacetyl-activated for the covalent immobilization of sulfhydryl-containing peptides or proteins for affinity purification.

SulfoLink Coupling Resin is porous, crosslinked 6% beaded agarose that has been activated with iodoacetyl groups. When incubated with a solution of peptide or protein that contains reduced cysteine residues, the iodoacetyl groups react specifically and efficiently with the exposed sulfhydryls (-SH) to form covalent and irreversible thioether bonds that permanently attach the peptide or protein to the resin. The result is a custom-made affinity resin for purification of antibodies, antigens and other molecules of interest.

Complete SulfoLink Immobilization Kits are available for peptides and proteins. Each kit contains all the reagents for preparing the respective peptide or protein sample and five columns containing 2mL of SulfoLink Resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification. A single-column Trial Kit for use with either peptides or proteins is also available, or the SulfoLink Coupling Resin can be purchased separately.

Highlights:

• Specific conjugation through sulfhydryl (-SH) groups – the iodoacetyl groups react specifically with sulhydryls to form irreversible thioether bonds

• Separate kits optimized for peptides or proteins – kits include optimized reagents for preparing peptide or protein samples for efficient immobilization

• Fast – spin columns increase protocol speed; prepare and couple samples in 2 hours (peptides) to 3.5 hours (proteins)

• Flexible coupling conditions – use pH 7.5 to 9.0 aqueous buffers, organic solvent (e.g., 20% DMSO) or denaturant (guanidine·HCl), as needed for protein or peptide solubility during coupling reaction

• Easy-to-follow instructions – streamlined protocols for sample preparation, immobilization and affinity purification

• High capacity – immobilize 1 to 2mg peptide or 2 to 20mg protein per 2mL column of SulfoLink Coupling Resin

Includes:

Kits contain SulfoLink Resin Columns (2mL), protein or peptide preparation reagents (e.g., reducing agent), coupling buffer, wash solution, L-cysteine solution (for quenching) and desalting columns (protein kit only)

Recommended for:

Immobilize peptides having terminal cysteine residues to purify antibodies that were generated against peptide immunogens prepared by maleimide conjugation; Immobilize antibodies in oriented manner through hinge-region sulfhydryls to ensure that antigen binding sites are not sterically hindered for antigen affinity purification

Specifications

• Capacity (Metric): 2 mL
• Product Line: SulfoLink™
• Type: Immobilization Kit
• Sufficient For: 1 to 2 mg of Sulfhydryl-Peptide per Column
• Column Type: Affinity, Agarose Resin

• Content And Storage: Sufficient For: Immobilizing 0.1 to 1 mg of sulfhydryl-peptide per column
  • SulfoLink Columns, 2 mL, 5 columns
  • SulfoLink Coupling Buffer, 120 mL
  • Wash Solution, 120 mL
  • PBS Pack (makes 500 mL), 1 pack
  • Bond-Breaker TCEP Solution, Neutral pH, 0.5 mL
  • L-Cysteine·HCl, 100 mg
  • Column Accessories
  
  Store at 4°C protected from light.
• Quantity: 5 Column Kit
• Stationary Phase: Haloacetyl
• Format: Spin Column

Safety and Handling

Product Identifier

• SulfoLink Immobilization Kit for Peptides, 2 mL

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H335-May cause respiratory irritation.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P310-Immediately call a POISON CENTER/doctor/.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : L-Cysteine, hydrochloride, hydrate

For Research Use Only. Not for use in diagnostic procedures.