IBA Lifesciences Strep-tag™ Protein Purification Buffer Set, IBA Lifesciences GmbH

Product Code. 12783064

Brand: IBA Lifesciences 21002001

Description

Strep-Tactin™ purification buffer set

Strep-Tactin™ Buffer Set contains all buffers necessary for purification of Strep-tag™II or Twin-Strep-tag™ fusion proteins with Strep-Tactin™ as well as for subsequent regeneration of the resin. Strep-Tactin™XT Buffer Set comprises the following buffers:
• 10x Buffer W, 100 ml
• 10x Buffer BXT, 25 ml
• 10x Buffer XT-R, 250 ml 10x Buffer W is a 10x concentrated wash buffer for Strep-Tactin™ and Strep-Tactin™XT resins. It removes unbound molecules from the affinity chromatography matrix during Strep-tag™II and Twin-Strep-tag™ fusion protein purification. 10x Buffer E is a 10x concentrated Strep-Tactin™ elution buffer. The contained desthiobiotin is a modified form of biotin that displaces Strep-tag™II and Twin-Strep-tag™ fusion proteins from the modified biotin-binding pocket of Strep-Tactin™ in a competitive manner, resulting in a mild elution. Please note that 10x Buffer E is not applicable for elution from Strep-Tactin™XT resins. 10x Buffer R is a 10x concentrated Strep-Tactin™ regeneration buffer containing HABA. HABA (2-[4-hydroxy-benzeneazo]benzoic acid) displaces desthiobiotin from the modified biotin-binding pocket of Strep-Tactin™ after the elution of Strep-tag™II or Twin-Strep-tag™ fusion proteins. The yellow buffer turns red upon binding of HABA to Strep-Tactin™, clearly indicating the regeneration process and activity status of the column.
Please note that the Strep-Tactin™ Buffer Set is not applicable for Strep-Tactin™XT.

Specifications

• Chemical Name or Material: Buffer W, Buffer E, Buffer R
• For Use With (Application): Purification of recombinant Strep-Tag fusion proteins on immobilized Strep-Tactin
• Quantity: 1 Kit
• Solution Type: Protein Purification Buffer

Safety and Handling

Product Identifier

• Strep-Tactin purification buffer set

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3
• Specific target organ toxicity after repeated exposure Category 2
• Acute toxicity Category 4

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H335-May cause respiratory irritation.
• H372-Causes damage to organs.

Precautionary Statement

• P264-Wash hands thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap and water.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.

For Research Use Only.