SSEA-5 Mouse anti-Human, Clone: 8E11-SSEA5, eBioscience™

Mouse Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 14-8857-82

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.01000kg



Disclaimers: For Research Use Only.

Product Code. 15218107

Quantity Price
1 £ 133.91 / 100µg
Estimated Shipment date
from Supplier 09-05-2017
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Description and Specification

Specification

Concentration 0.5mg/mL
Applications Flow Cytometry
Applications Immunocytochemistry
Applications Western Blotting
Isotype IgG2b
Host Species Mouse
Clone 8E11-SSEA5
Antigen SSEA-5
Primary or Secondary Primary
Regulatory Status RUO
Format Purified
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Gene Alias Stage-Specific Embryonic Antigen-5
Quantity 100μg
Monoclonal or Polyclonal Monoclonal
Conjugate Unlabeled
Species Reactivity Human
Storage Requirements Store at 2-8°C.

The monoclonal antibody 8E11-SSEA5 recognizes human stage-specific embryonic antigen-5 (SSEA-5), an H type-1 glycan. SSEA-5 belongs to the SSEA family of glycoproteins and is highly expressed on pluripotent human stem cells including embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. During embryonic development, SSEA-5 is expressed within the blastocyst on inner cell mass (ICM) cells that later give rise to ES cells. As ES and iPS cells differentiate, SSEA-5 expression is downregulated. The tumorigenic potential of contaminating stem cells in cell populations for transplantation is a hurdle to using this approach for disease therapy. The 8E11 antibody in combination with either CD9 and CD90 or CD50 and CD200 to sort populations has been shown to effectively separate teratoma-forming cells from stem cell-derived populations of differentiated cells.