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SSEA4 Mouse anti-Canine, Chicken, Human, Mouse, Non-human primate, Rabbit, Alexa Fluor™ 488, Clone: eBioMC-813-70 (MC-813-70), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 53-8843-41

Code : NEW

Additional Details : Weight : 0.23750kg

 View more versions of this product

Product Code. 15570257

  • £109.00 / 25 tests

Estimated Shipment
14-05-2021

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Description

Description

Description: The eBioMC-813-70 monoclonal antibody reacts with a glycolipid carbohydrate epitope on Stage-specific embryonic antigen-4 (SSEA-4) protein. Expression is found on human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), human induced pluripotent stem (iPS) cells, and human embryonic stem cells (ES) with a decrease in expression upon differentiation. Recently expression has also been found on bone marrow mesenchymal stem cells. The eBioMC-813-70 antibody has been reported to crossreact to mouse, rabbit, canine, chicken, and Old and New World monkeys. Applications Reported: This eBioMC-813-70 (MC-813-70) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioMC-813-70 (MC-813-70) antibody has been pre-titrated and tested by flow cytometric analysis of the 2102Ep cell line. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

SSEA-4 (Stage-specific embryonic antigen 4) is a cell surface antigen, expressed along with SSEA-3, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA-1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.
Specifications

Specifications

SSEA4
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
stage-specific embryonic antigen 4; SSEA-4
IgG3
25 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
Canine, Chicken, Human, Mouse, Non-human primate, Rabbit
Flow Cytometry
eBioMC-813-70 (MC-813-70)
Alexa Fluor™ 488
Liquid
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Videos
SDS
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For Research Use Only.