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SSEA1 Mouse anti-Human, Mouse, Alexa Fluor™ 488, Clone: eBioMC-480 (MC-480), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 53-8813-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15540257

  • £250.00 / 100 tests

Estimated Shipment: 05-10-2021

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Description

Description

Description: The eBioMC-480 (MC-480) antibody reacts with the stage-specific embryonic antigen-1 (SSEA-1), a carbohydrate epitope expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine and human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Differentiation of human EC results in an increase in SSEA-1 expression, while in the mouse expression is diminished. Expression of the carbohydrate moiety is also found on mature human granulocytes (on CD15) and some monocytes. SSEA-1 is associated with cell adhesion, migration and differentiation. Applications Reported: This eBioMC-480 (MC-480) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioMC-480 (MC-480) antibody has been pre-titrated and tested by flow cytometric analysis of F9 cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determit (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.
Specifications

Specifications

SSEA1
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
stage-specific embryonic antigen 1; CD15; SSEA-1; SSEA1; ELFT; FCT3A; FUC-TIV; FUTIV; LeX
IgM
100 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
Human, Mouse
Flow Cytometry
eBioMC-480 (MC-480)
Alexa Fluor 488
Liquid
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
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For Research Use Only.