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SOX2, eFluor 570, clone: Btjce, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 41-9811-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15578976

  • £83.79 / 25µg


Estimated Shipment date
from Supplier 31-05-2018
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Description

Description

Description: The Btjce monoclonal antibody reacts with the transcription factor Sox2, a member of the SOX (sex determining region Y -related HMG (High Mobility Group) Box) family of proteins. Sox family members play a role in early organ development, and in particular, Sox2 is essential for regulating genes that control normal mammalian embryogenesis. Sox2 and family member Sox3 are expressed as early as the preimplantation and epiblast stages respectively. Later expression is restricted to the neuroepithelium. Sox2 has been shown to be necessary for maintaining self-renewal and pluripotency of mouse and human embryonic stem (ES) cells (ESC). Oct4 (POU5F1), Klf4, c-myc, and Sox2 were the original four factors used to reprogram differentiated mouse and human cells to induced pluripotent stem cells (iPSC).Expression of Sox2 is tightly regulated and recent studies have demonstrated that small changes in the levels of Sox2 in ES cells can trigger differentiation into multiple cell types. Sox2 expression is not limited to ES cells, it is also essential for early neurogenesis where its expression becomes restricted to the neural plate, and later to neural stem cells where it functions to suppress neural differentiation. Sox2 in combination with other stem cell markers can be used to characterize stem cell populations. Ectopic expression of Sox2 has been associated with multiple cancer types including colorectal and breast.Applications Reported: This Btjce antibody has been reported for use in microscopy, immunohistochemical staining, and immunocytochemistry.Applications Tested: This Btjce antibody has been tested by immunocytochemistry of fixed and permeabilized F9 cells. Thsi can be used at less than 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.Excitation: 555 nm; Emission: 570 nm
Specifications

Specifications

Sox2
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P48431, P48432
ANOP3, MCOPS3, SRY-related HMG-box gene 2, transcription factor SOX-2, transcription factor SOX2, sex determining region Y box 2
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Microscopy
Btjce
eFluor 570
SOX2
Conjugated
SOX2
IgG2a, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
6657
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