SOX10, clone: 20B7, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-5923-80
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionDescription: The monoclonal antibody 20B7 recognizes Sox10, is a member of the Sox superfamily (SRY-related HMG-box) that contain a DNA binding domain (high-mobility group domain) and functions as a transcription factor. Initially, Sox10 expression is localized to the developing neural crest and peripheral nervous system, but later is expressed in glial progenitors and mature oligodendrocytes. Sox10 is involved in stem cell maintenance, melanocyte differentiation, as well as central and peripheral gliogenesis and neurogenesis. Sox10 regulation of downstream proteins such as MASH1, PHOX2B, PDGF-alpha, and MITF is dependent on expression level. Sox10 is also expressed in multipotent vascular stem cells, melanomas, and gliomas. The 20B7 antibody is reported to recognize human and rat Sox 10, and based on homology should recognize mouse Sox10.Applications Reported: This 20B7 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.Applications Tested: This 20B7 antibody has been tested by immunocytochemistry of formaldehyde fixed and permeabilized rat cells. This 20B7 antibody has also been tested by immunohistochemistry of human formalin-fixed paraffin embedded tissue sections using low pH antigen retreival and can be used at less than or equal to 10 ug/mL for both applications. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|P56693, Q04888, O55170|
|SRY, sex determining region Y-box 10 , DOM, MGC15649, WS4, SRY-related HMG-box gene 10|
|Store at 2-8°C.|
For Research Use Only.