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SAP (SLAM-Associated Protein), PE, clone: XLP-1D12, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-9787-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15597226

  • £217.51 / 100 tests


EU Stock 5
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Description

Description

Description: The monoclonal antibody XLP-1D12 reacts with human SLAM-associated protein (SAP), which is also known as SH2D1A. SAP is a 15-kDa SH2 domain-containing adaptor protein. Upon CD150/SLAM family receptor ligation, SAP recruits Fyn tyrosine kinase, which phosphorylates the receptor to activate downstream signaling events. SAP is expressed in thymocytes, T cells, NK cells, and NKT cells. Studies have demonstrated a role for SAP in promoting follicular T helper cell differentiation during germinal center formation. In addition, this protein is involved in NKT cell development, Th1/Th2 differentiation, development of innate T cells, and regulation of NK cell and CD8+ T cell cytotoxicity. Mutations of the gene encoding SAP results in X-linked lymphoproliferative (XLP) syndrome, which is characterized by impaired humoral immunity leading to increased susceptibility to Epstein-Barr virus infection and/or lymphoma. SAP has also been linked to automimmune diseases such as systemic lupus erythematosus.Applications Reported: This XLP-1D12 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This XLP-1D12 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transicription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources tab online. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

SLAM Associated Protein (SAP)
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
O60880
DSHP, EBVS, IMD5, LYP, MTCP1, SH2D1A, XLP, XLPD
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
XLP-1D12
PE
SH2D1A
Conjugated
SH2D1A
IgG2a, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
4068
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