Sino Biological™ TNFRSF1B Recombinant Human Protein, hIgG1-Fc.His Tag

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Asp 257) of human TNFRSF1B (NP_001057.1) fused to the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus

Overview
Brand: Invitrogen

Manufacturer Part Number: 10417H03H25

RECOM HU TNFRSF1B (HIGG1-FC-HI

UNSPSC: 12352202

Code: NEW

Additional Details:
Additional Details: Weight: 0.10000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 15295375

Quantity Price
1 £ 121.0 / 25µg
Estimated Shipment date
from Supplier 13-12-2016
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Description and Specification

Specification

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Molecular Weight 53kDa
Purity ≥95% by SDS-PAGE
Quantity 5 x 5μg
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Protein Subtype TNF Receptors
Endotoxin Concentration <1 EU/μg
Gene Alias TNFRSF1B, CD120b, TBPII, TNF-R-II, TNF-R75, TNFBR, TNFR1B, TNFR2, TNFR80, p75TNFR
Common Name Tumor necrosis factor receptor superfamily, member 1b
Gene Symbol TNFRSF1B
Protein Tag His Tag/IgG1 Fc Tag
Validated Application Assay Standard
Protein Family Cytokines & Receptors/CD Proteins
Expression System Mammalian

Tumor Necrosis Factor Receptor Superfamily Member 1B (TNFRSF1B), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.
  • Activity: Measured by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, the ED50 for this effect is typically 4-16ng/mL in the presence of 0.25ng/mL recombinant human TNF-alpha
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.