Sino Biological™ TNFRSF1A (CD120a) Recombinant Mouse Protein, His Tag

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Ala 212) of mouse TNFRSF1A (NP_ 035739.2) fused to a polyhistidine tag at the C-terminus

Overview
Brand: Invitrogen

Manufacturer Part Number: 50496M08H25

TNFRSF1A (CD120a) Recombinant Mouse Protein (His

UNSPSC: 12352202

Code: NEW

Additional Details:
Additional Details: Weight: 0.01000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 13496306

Quantity Price
1 £ 144.0 / 25µg
EU Stock 3
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Description and Specification

Specification

Quantity 5 x 5μg
Protein Subtype TNF (Tumor Necrosis Factor)
Common Name Tumor necrosis factor receptor superfamily, member 1A
Protein Tag His Tag
Protein Family Cytokines & Receptors/CD Proteins
For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Molecular Weight 21.8kDa
Purity ≥90% by SDS-PAGE
Storage Requirements Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Structural Form Truncated/Recombinant
Endotoxin Concentration <1 EU/μg
Gene Alias Tnfrsf1, CD120a, FPF, TNF-R, TNF-R-I, TNF-R1, TNF-R55, TNF-alphaR1, TNFAR, TNFR60, TNFRI
Gene Symbol TNFRSF1A
Validated Application Assay Standard
Expression System Mammalian
Format Lyophilized
Gene ID (Entrez) 21937

Tumor necrosis factor receptor superfamily member 1A (TNFRSF1A/CD120a), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.
  • Activity: 1. Measured by its binding ability in a functional ELISA: a. Immobilized human TNFA at 10μg/mL (100μL/well) can bind biotinylated mouse TNFRSF1A-his, the ED50 of biotinylated mouse TNFRSF1A-his is 0.28μg/mL; b. Immobilized mouse TNFA at 10μg/mL (100μL/well) can bind biotinylated mouse TNFRSF1A-his, the ED50 of biotinylated mouse TNFRSF1A-his is 0.13μg/mL; 2. Measured by its ability to inhibit the TNF mediated cytotoxicity in the L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, the ED50 for this effect is typically 0.5-1.5μg/mL in the presence of 0.1ng/mL recombinant mouse TNF
  • Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

Shipping Condition: Room Temperature. This protein is manufactured by Sino Biological Inc.