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Thermo Scientific™ Sino Biological™ TNFRSF1A (CD120a) Recombinant Mouse Protein, His Tag

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Ala 212) of mouse TNFRSF1A (NP_ 035739.2) fused to a polyhistidine tag at the C-terminus

Brand:  Thermo Scientific™ 50496M08H25

Code : NEW

Additional Details : Weight : 0.01000kg

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Product Code. 13496306

  • £144.00 / 25µg


EU Stock 3
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Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Description

Description

Tumor necrosis factor receptor superfamily member 1A (TNFRSF1A/CD120a), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.
  • Activity: 1. Measured by its binding ability in a functional ELISA: a. Immobilized human TNFA at 10μg/mL (100μL/well) can bind biotinylated mouse TNFRSF1A-his, the ED50 of biotinylated mouse TNFRSF1A-his is 0.28μg/mL; b. Immobilized mouse TNFA at 10μg/mL (100μL/well) can bind biotinylated mouse TNFRSF1A-his, the ED50 of biotinylated mouse TNFRSF1A-his is 0.13μg/mL; 2. Measured by its ability to inhibit the TNF mediated cytotoxicity in the L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, the ED50 for this effect is typically 0.5-1.5μg/mL in the presence of 0.1ng/mL recombinant mouse TNF
  • Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

Shipping Condition: Room Temperature. This protein is manufactured by Sino Biological Inc.

Specifications

Specifications

Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
21937
≥90% by SDS-PAGE
Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
TNF (Tumor Necrosis Factor)
Tnfrsf1, CD120a, FPF, TNF-R, TNF-R-I, TNF-R1, TNF-R55, TNF-alphaR1, TNFAR, TNFR60, TNFRI
TNFRSF1A
Cytokines & Receptors/CD Proteins
His Tag
Lyophilized
21.8kDa
5 x 5μg
Truncated/Recombinant
<1 EU/μg
Tumor necrosis factor receptor superfamily, member 1A
Assay Standard
Mammalian
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