Thermo Scientific™ Sino Biological™ TNFRSF1A (CD120a) Recombinant Human Protein, His Tag
This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Thr 211) of human TNFRSF1A (NP_001056.1) fused to a polyhistidine tag at the C-terminus.
Brand: Thermo Scientific™ 10872H08H250
Code : NEW
Additional Details : Weight : 0.01000kg
DescriptionTumor necrosis factor receptor superfamily member 1A (TNFRSF1A/CD120a), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.
- Activity: 1. Measured by its binding ability in a functional ELISA; a. Immobilized human TNFa at 10μg/mL (100μL/well) can bind biotinylated human TNFRSF1A-his, the ED50 of biotinylated human TNFRSF1A-his is 0.04μg/mL; b. Immobilized mouse TNFa at 10μg/mL (100μL/well) can bind biotinylated human TNFRSF1A-his, the ED50 of biotinylated human TNFRSF1A-his is 0.28μg/mL; 2. Measured by its ability to inhibit the TNF mediated cytotoxicity in the L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, the ED50 for this effect is typically 0.04-0.2μg/mL in the presence of 0.25ng/mL recombinant human TNF
- Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80
- Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible
Shipping Condition: Room Temperature. This protein is manufactured by Sino Biological Inc.
|Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories|
|≥90% by SDS-PAGE|
|Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.|
|TNF (Tumor Necrosis Factor)|
|CD120a, FPF, mgC19588, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR60, p55, p55-R, p60|
|Cytokines & Receptors/CD Proteins|
|5 x 50μg|
|Tumor necrosis factor receptor superfamily, member 1A|