Sino Biological™ TNF (TNFA) Recombinant Rat Protein

This recombinant protein was expressed from a DNA sequence encoding the mature form of rat TNF-α (P 16599) (Val 80-Leu 235) fused to an initial Met at the N-terminus

Brand: Invitrogen

Manufacturer Part Number: 80045RNAE50

UNSPSC: 12352202

Code: NEW

Additional Details:
Additional Details: Weight: 0.01000kg

Disclaimers: For Research Use Only. Not for use in diagnostic procedures.
Includes: One vial of lyophilized protein

Product Code. 14840841

Quantity Price
1 £ 231.0 / 50µg
Estimated Shipment date
from Supplier 15-05-2017
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Description and Specification


Format Lyophilized
Gene Symbol TNF
Quantity 50μg
For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Expression System E. coli
Protein Tag Untagged
Protein Family Cytokines & Receptors
Protein Subtype TNF (Tumor Necrosis Factor)
Gene Alias Tnf, mgC124630, RATTNF, TNF-alpha, Tnfa
Purity ≥90% by SDS-PAGE
Common Name Tumor necrosis factor
Molecular Weight 17.4kDa
Validated Application Assay Standard

Tumor Necrosis Factor (TNF), recombinant rat protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.
  • Activity: Measured in a cytotoxicity assay using L930 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D; the ED50 is typically 10-20pg/mL
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.