Sino Biological™ TNF (TNFA) Recombinant Cynomolgus Protein

This recombinant protein was expressed from a DNA sequence encoding the soluble form of cynomolgus TNF-α (Val 77-Leu 233) fused to an initial Met at the N-terminus

Overview
Brand: Invitrogen

Manufacturer Part Number: 90018CNAE5

RECOM C TNFA

Code: NEW

Additional Details:
Additional Details: Weight: 0.10000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.
Includes: One vial of lyophilized protein

Product Code. 15309696

Quantity Price
1 £ 54.0 / 5µg
Estimated Shipment date
from Supplier 09-12-2016
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Description and Specification

Specification

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Molecular Weight 17.4kDa
Purity ≥90% by SDS-PAGE
Quantity 5μg
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Protein Subtype TNF (Tumor Necrosis Factor)
Gene Alias TNF-alpha
Common Name Tumor necrosis factor
Gene Symbol TNF
Protein Tag Untagged
Validated Application Assay Standard
Protein Family Cytokines & Receptors
Expression System E. coli

Tumor Necrosis Factor (TNF), recombinant cynomolgus protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.
  • Activity: Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D; the ED50 is typically 20-80pg/mL
  • Formulation: Lyophilized in 50mM Tris, 50mM NaCl, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.