Sino Biological™ TFPI Recombinant Human Protein, His Tag

This recombinant protein was expressed from a DNA sequence encoding the human TFPI (NP_006278.1) (Met 1-Lys 282) fused to a C-terminal polyhistidine tag

Overview
Brand: Invitrogen

Manufacturer Part Number: 10564H08H50

TFPI RECOMBINANT HUMAN PROTEIN, HIS TAG

UNSPSC: 12352202

Code: NEW

Additional Details:
Additional Details: Weight: 0.10000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 15246935

Quantity Price
1 £ 746.0 / 50µg
EU Stock 3
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Description and Specification

Specification

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Molecular Weight 30.6kDa
Purity >85% by SDS-PAGE
Quantity 50μg
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Protein Subtype Proteinase Inhibitor
Endotoxin Concentration <1 EU/μg
Gene Alias EPI, LACI, TFI, TFPI1
Common Name Tissue factor pathway inhibitor (liporeotein-associated coagulation inhibitor)
Gene Symbol TFPI
Protein Tag His Tag
Validated Application Assay Standard
Protein Family Protease Inhibitors
Expression System Mammalian

Tissue Factor Pathway Inhibitor (Liporeotein-Associated Coagulation Inhibitor) (TFPI), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as enzymatic analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.
  • Activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec Cat. No.27114), the IC50 value is <0.4nM, as measured in 100μL reaction mixture containing 1.25ng trypsin (Sigma Cat. No.T4799), 10μM substrate, 50mM Tris, 10mM CaCl2, 0.15M NaCl, 0.05% Brij-35, pH 7.5
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.