SPINT1 Recombinant Human Protein, His Tag, Invitrogen™ Sino Biological™

This recombinant protein was expressed from a DNA sequence encoding the human SPINT1 (O43278-2) (Met 1-Val 433) fused to a polyhistidine tag at the C-terminus

Product Code	Brand	Quantity
15249095	Invitrogen 11742H08H5	5μg
15229095	Invitrogen 11742H08H25	5 x 5μg

Description

Serine Peptidase Inhibitor Kazal Type 1 (SPINT1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as enzymatic analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

• Activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH₂ (R&D Systems Cat. No. ES002); the IC50 value is <2nM
• Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na₂HPO₄, 1.8mM KH₂PO₄, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
• Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.

Specifications

• For Use With (Application): Protein Expression and Isolation, Enzyme and Protein Activity Assay, Protein Assay Control, Reference Standards and Accessories
• Storage Requirements: Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
• Gene Alias: SPINT1, UNQ223/PRO256, HAI, HAI1, MANSC2
• Gene Symbol: SPINT1
• Expression System: Mammalian
• Form: Lyophilized
• Purity or Quality Grade: ≥90% by SDS-PAGE

• Molecular Weight (g/mol): 45.8kDa
• Endotoxin Concentration: <1 EU/μg
• Common Name: Serine peptidase inhibitor, Kazal type 1
• Protein Tag: His Tag
• Protein Family: Protease Inhibitors
• Protein Subtype: Protease Inhibitors

For Research Use Only. Not for use in diagnostic procedures.