Thermo Scientific™ Sino Biological™ SERPINA1 Recombinant Human Protein, His Tag
This recombinant protein was expressed from a DNA sequence encoding the human SERPINA1 pre-protein (NP_000286.3) (Met 1-Lys 418) fused to the polyhistidine tag at the C-terminus
Brand: Thermo Scientific™ 10306H08H50
Code : NEW
Additional Details : Weight : 0.10000kg
DescriptionSerpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.
- Activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2
; the IC50 value is <3.0nM, as measured in 100μL reaction mixture containing 1.25ng trypsin, 10μM substrate, 50mM Tris, 10mM CaCl2, 0.15 M NaCl, pH 7.5
- Formulation: Lyophilized in 25mM Tris, 150mM NaCl, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
- Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible
This protein is manufactured by Sino Biological Inc.
|Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories|
|SerpinA1, PI, A1A, AAT, PI1, A1AT, mgC9222, PRO2275, mgC23330|
|≥90% by SDS-PAGE|
|Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.|
|Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1|