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Sino Biological™ S100A9 Recombinant Human Protein, His Tag

This recombinant protein is expressed from a DNA sequence encoding the human S100A9 (NP_002956.1) (Met 1-Pro 114) fused to a polyhistidine tag at the C-terminus

Brand:  Invitrogen 11145H08B250

UNSPSC : 12352202

Code : NEW

Additional Details : Weight : 0.10000kg

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Product Code. 15288295

  • £622.00 / 250µg


Estimated Shipment
11-08-2017
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Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Specifications

Description

S100 Calcium Binding Protein A9 (S100A9) recombinant human protein is supplied as a lyophilized powder. This protein is suitable for use in performing cell-based assays and protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.&line-break;Background: Human Protein S100-A9, also known as S100 calcium-binding protein A9, is a calcium-binding protein. It has anti-microbial activity towards bacteria and fungi. The anti-microbial and proapoptotic activity of S100A9 is inhibited by zinc ions. S100A9 plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS). It promotes tubulin polymerization when unphosphorylated. It also promotes phagocyte migration and infiltration of granulocytes at sites of wounding. S100A9 plays a role as a proinflammatory mediator in acute and chronic inflammation and upregulates the release of IL8 and cell-surface expression of ICAM1.
  • N-terminal Sequence Analysis: Met
  • Activity: 1. Measured by its ability to bind recombinant human S100A8-his in a functional ELISA; 2. Measured by its ability to bind recombinant human his-S100A8 in a functional ELISA; 3. Measured by its ability to inhibit the proliferation of MCF7 human breast adenocarcinoma cells; the ED50 for this effect is typically 10-30μg/mL
  • Formulation: lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% mannitol, 5% trehalose, 0.02% Tween®-80
  • Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

Manufactured by Sino Biological (China).

Description & Specifications

Specifications

Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
14.6kDa
5 x 50μg
Other Proteins
60B8AG, CAGB, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14, NIF, P14
S100A9
Other Proteins
His Tag
Lyophilized
≥90% by SDS-PAGE
Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
<1 EU/μg
S100 calcium binding protein A9
Assay Standard
Insect