Sino Biological™ PECAM1 Recombinant Human Protein, hIgG1-Fc Tag

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Lys 601) of human PECAM1 (EAW94208.1) fused to the fused Fc region of human IgG1 at the C-terminus

Overview
Brand: Invitrogen

Manufacturer Part Number: 10148H02H5

RECOM HU PECAM1

UNSPSC: 12352202

Code: NEW

Additional Details:
Additional Details: Weight: 0.10000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 15294735

Quantity Price
1 £ 53.25 / 5µg
Estimated Shipment date
from Supplier 08-12-2016
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Description and Specification

Specification

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Molecular Weight 91.2kDa
Purity ≥90% by SDS-PAGE
Quantity 5μg
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Protein Subtype Intercellular Adhesion Molecule
Endotoxin Concentration <1 EU/μg
Gene Alias PECAM1, CD31, PECAM-1
Common Name Platelet/endothelial cell adhesion molecule 1
Gene Symbol PECAM1
Protein Tag IgG1 Fc Tag
Validated Application Assay Standard
Protein Family CD Proteins
Expression System Mammalian

Platelet/Endothelial Cell Adhesion Molecule 1 (PECAM1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.
  • Activity: Measured by its ability of the immobilized protein to support the adhesion of Jurkat human leukemic T cells; when cells are added to recombinant human PECAM1 coated plates (3.3μg/mL, 100μL/well) in the presence of PMA, approximately 50%-65% will adhere specifically after 30 minutes at 37°C
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.