Sino Biological™ MET (c-MET) Recombinant Human Protein (without Catalytic Activity Domain), His Tag

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Thr 932) of human MET (NP_000236) fused to a polyhistidine tag at the C-terminus

Overview
Brand: Invitrogen

Manufacturer Part Number: 10692H08H50

UNSPSC: 12352202

Code: NEW

Additional Details:
Additional Details: Weight: 0.01000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 13455926

Quantity Price
1 £ 228.0 / 50µg
Estimated Shipment date
from Supplier 26-05-2017
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Description and Specification

Specification

Format Lyophilized
KinaseFamily MET (HGFR) Kinase Family
Purity ≥85% by SDS-PAGE
Protein Tag His Tag
Gene Alias MET, AUTS9, HGFR, RCCP2, c-Met
Structural Form Truncated/Recombinant
Endotoxin Concentration <1 EU/μg
Protein Family Growth Factors & Receptors
Storage Requirements Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Expression System Mammalian
Quantity 50μg
Molecular Weight 103.0kDa
Validated Application Assay Standard
For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Protein Subtype Receptor Tyrosine Kinases|Growth Factor Receptors
Gene ID (Entrez) 4233
Common Name Met proto-oncogene
Gene Symbol MET

Met proto-oncogene/hepatocyte growth factor receptor (MET/c-MET), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. Since this product is a truncated protein and does not contain intracellular catalytic activity domain, it is not suitable for use in enzyme activity studies.
  • Activity: Measured by its binding ability in a functional ELISA; immobilized recombinant human HGF at 10μg/mL (100μL/well) can bind biotinylated MET, the ED50 of biotinylated Met is 5.28μg/mL
  • Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween-80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

Shipping Condition: Room Temperature. This protein is manufactured by Sino Biological Inc.