MET (c-MET) Recombinant Human Protein (without Catalytic Activity Domain), His Tag, Invitrogen™ Sino Biological™

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Thr 932) of human MET (NP_000236) fused to a polyhistidine tag at the C-terminus

Brand:  Invitrogen 10692H08H250

Product Code. 13435926

Description

Met proto-oncogene/hepatocyte growth factor receptor (MET/c-MET), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. Since this product is a truncated protein and does not contain intracellular catalytic activity domain, it is not suitable for use in enzyme activity studies.

• Activity: Measured by its binding ability in a functional ELISA; immobilized recombinant human HGF at 10μg/mL (100μL/well) can bind biotinylated MET, the ED₅₀ of biotinylated Met is 5.28μg/mL
• Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween-80
• Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

Shipping Condition: Room Temperature. This protein is manufactured by Sino Biological Inc.

Specifications

• For Use With (Application): Protein Expression and Isolation, Enzyme and Protein Activity Assay, Protein Assay Control, Reference Standards and Accessories
• Molecular Weight (g/mol): 103.0kDa
• Storage Requirements: Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
• Endotoxin Concentration: <1 EU/μg
• Common Name: Met proto-oncogene
• KinaseFamily: MET (HGFR) Kinase Family
• Expression System: Mammalian
• Form: Lyophilized
• Purity or Quality Grade: ≥85% by SDS-PAGE

• Gene ID (Entrez): 4233
• Quantity: 5 x 50μg
• Structural Form: Truncated/Recombinant
• Gene Alias: MET, AUTS9, HGFR, RCCP2, c-Met
• Gene Symbol: MET
• Protein Tag: His Tag
• Protein Family: Growth Factors & Receptors
• Protein Subtype: Receptor Tyrosine Kinases, Growth Factor Receptors

For Research Use Only. Not for use in diagnostic procedures.