Sino Biological™ MCAM Recombinant Human Protein, His Tag

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of human MCAM precursor (NP_006491.2) (Met 1-Gly 559) fused to a C-terminal polyhistidine tag

Overview
Brand: Invitrogen

Manufacturer Part Number: 10115H08H5

UNSPSC: 12352202

Code: NEW

Additional Details:
Additional Details: Weight: 0.10000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 15234675

Quantity Price
1 £ 133.0 / 5µg
Estimated Shipment date
from Supplier 15-05-2017
Add to basket


Description and Specification

Specification

Protein Subtype Intercellular Adhesion Molecule
Molecular Weight 61.3kDa
For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Validated Application Assay Standard
Purity ≥90% by SDS-PAGE
Protein Family CD Proteins
Gene Symbol MCAM
Gene Alias MCAM, CD146, MUC18
Common Name Melanoma Cell Adhesion Molecule
Quantity 5μg
Endotoxin Concentration <1 EU/μg
Format Lyophilized
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.Expires one year from date of receipt when stored as instructed.
Protein Tag His Tag
Expression System Mammalian

Melanoma Cell Adhesion Molecule (MCAM), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.
  • Activity: Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line; when 3 x 104 cells/well are added to human MCAM coated plates (0.8μg/mL, 100μL/well), approximately >50% will adhere after one hour at 37°C
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.